In-Yeast Meeting of Coronavirus Infectious cDNA Clones Utilizing a Artificial Genomics Pipeline
The Escherichia coli and vaccinia virus-based reverse genetics methods have been broadly utilized for the manipulation and engineering of coronavirus genomes. These methods, nonetheless, current a number of limitations and are generally tough to determine in a well timed method for (re-)rising viruses. On this chapter, we current a brand new common reverse genetics platform for the meeting and engineering of infectious full-length cDNAs utilizing yeast-based transformation-associated recombination cloning.
This novel meeting technique not solely ends in secure coronavirus infectious full-length cDNAs cloned within the yeast Saccharomyces cerevisiae but additionally fosters and accelerates the manipulation of their genomes.
Such a platform is broadly relevant for the scientific neighborhood, because it requires no particular gear and could be carried out in an ordinary laboratory setting. The protocol described could be simply tailored to just about all recognized or rising coronaviruses, reminiscent of Center East respiratory syndrome coronavirus (MERS-CoV).
RNA-cDNA hybrids mediate transposition via different mechanisms
Retrotransposons can represent half of eukaryotic genomes. Retrotransposon dysregulation destabilizes genomes and has been linked to various human diseases. Emerging regulators of retromobility include RNA-DNA hybrid-containing structures known as R-loops. Accumulation of these structures at the transposons of yeast 1 (Ty1) elements has been shown to increase Ty1 retromobility through an unknown mechanism.
Here, via a targeted genetic screen, we identified the rnh1Δ rad27Δ yeast mutant, which lacked both the Ty1 inhibitor Rad27 and the RNA-DNA hybrid suppressor Rnh1. The mutant exhibited elevated levels of Ty1 cDNA-associated RNA-DNA hybrids that promoted Ty1 mobility. Moreover, in this rnh1Δ rad27Δ mutant, but not in the double RNase H mutant rnh1Δ rnh201Δ, RNA-DNA hybrids preferentially existed as duplex nucleic acid structures and increased Ty1 mobility in a Rad52-dependent manner.
The data indicate that in cells lacking RNA-DNA hybrid and Ty1 repressors, elevated levels of RNA-cDNA hybrids, which are associated with duplex nucleic acid structures, boost Ty1 mobility via a Rad52-dependent mechanism.
In contrast, in cells lacking RNA-DNA hybrid repressors alone, elevated levels of RNA-cDNA hybrids, which are associated with triplex nucleic acid structures, boost Ty1 mobility via a Rad52-independent process. We propose that duplex and triplex RNA-DNA hybrids promote transposon mobility via Rad52-dependent or -independent mechanisms.
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Identification of salinity responsive genes in lavender through cDNA-AFLP
Currently, a global demand exists forlavender as a significant medicinal plant and source of essential oils. Freshwater and arable lands are two major factors that inhibit extensive farming of medicinal plants in Iran. Saline water from seas and salty soil may be new resources for agricultural use, especially for medicinal plants.
We sought to extend our knowledge of the Lavandula angustifolia genome and molecular basis of its salinity tolerance by using cDNA amplified fragment length polymorphism (cDNA-AFLP) to investigate the changes in plant transcriptomes in response to NaCl.
All identified transcript derived fragments (TDF) were assigned as novel L. angustifolia genes related to signal transduction, regulation of gene expression, alternative splicing, autophagy, and secondary metabolite biosynthesis. qRT-PCR analysis of the TDFs in response to different concentrations of NaCl revealed various levels of mRNA of the identified genes in this plant. Our findings provided primary insights into the molecular response of L. angustifolia to salinity.
piggenome
(untagged)-Human mRNA full length insert cDNA clone EUROIMAGE 1679989
Description: Human recombinant histone octamer consisting of 2 molecules each of histones H2A (a.a. 1-130(end)), H2B (a.a. 1-126(end)), H3 (a.a. 1-136(end)), and H4 (a.a. 1-103(end)), expressed in an E. coli expression system. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Global MW = 110 kDa._x000D_
Description: Recombinant full-length human SYK (GenBank accession #NM_003177) with an N-terminal GST-tag was expressed in Sf9 insect cells via a baculovirus expression system. MW = 10 kDa.
Description: Human SMO Full Length Protein-VLP (SMO-H52P3) is expressed from human 293 cells (HEK293). It contains AA Arg 28 - Phe 787 (Accession # Q99835-1).
Description: Human CD37 Full Length Protein (VLP) (CD7-H52P4) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Arg 281 (Accession # P11049).
Description: Human CD20 Full Length Protein-VLP (CDP-H52P6) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Pro 297 (Accession # P11836-1).
Description: Human CD81 Full Length Protein-VLP (CD1-H52P5) is expressed from human 293 cells (HEK293). It contains AA Gly 2 - Tyr 236 (Accession # P60033).
Description: Human DRD1 Full Length Protein (VLP) (DR1-H52P6) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Thr 446 (Accession # P21728-1).
Description: Human CCR5 Full Length Protein-VLP (CC5-H52P3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Leu 352 (Accession # P51681-1).
Description: Human CCR8 Full Length Protein-VLP (CC8-H52P4) is expressed from human 293 cells (HEK293). It contains AA Asp 2 - Leu 355 (Accession # P51685-1).
Description: Human CNR1 Full Length Protein-VLP (CN1-H52P6) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Lys 472 (Accession # P21554-1 (T210A)).
Description: Human CNR2 Full Length Protein (VLP) (CN2-H52P5) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Cys 360 (Accession # P34972-1).
Description: Human PAR1 Full Length Protein-VLP (PA1-H52P6) is expressed from human 293 cells (HEK293). It contains AA Ser 42 - Thr 425 (Accession # P25116-1).
Description: Human PAR2 Full Length Protein (VLP) (PA2-H52P7) is expressed from human 293 cells (HEK293). It contains AA Ser 37 - Tyr 397 (Accession # P55085).
Description: Human PAR2 Full Length Protein (VLP) (PA2-H52P7) is expressed from human 293 cells (HEK293). It contains AA Ser 37 - Tyr 397 (Accession # P55085).
Description: Human PAR2 Full Length Protein (VLP) (PA2-H52P7) is expressed from human 293 cells (HEK293). It contains AA Ser 37 - Tyr 397 (Accession # P55085).
Description: Human PAR2 Full Length Protein (VLP) (PA2-H52P7) is expressed from human 293 cells (HEK293). It contains AA Ser 37 - Tyr 397 (Accession # P55085).
Description: Human SMYD3 (GenBank Accession No. BC031010), full length, with N-terminal FLAG-tag, MW=50 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Description: Human CD133 Full Length-VLP (CD3-H52P3) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - His 865 (Accession # O43490-1).
Description: Human CXCR3 Full Length Protein-VLP (CX3-H52P4) is expressed from human 293 cells (HEK293). It contains AA Val 2 - Leu 368 (Accession # P49682-1).
Description: Mouse CXCR3 Full Length Protein (VLP) (CX3-M52P7) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Leu 367 (Accession # O88410).
Description: Human CXCR5 Full Length Protein-VLP (CX5-H52P4) is expressed from human 293 cells (HEK293). It contains AA Asn 2 - Phe 372 (Accession # P32302-1).
Description: Human GPR56 Full Length Protein-VLP (GP6-H52P7) is expressed from human 293 cells (HEK293). It contains AA Arg 26 - Ile 693 (Accession # Q9Y653-1).
Description: Human GPR87 Full Length Protein (VLP) (GP7-H52P3) is expressed from human 293 cells (HEK293). It contains AA Gly 2 - Val 358 (Accession # Q9BY21).
Description: Human EDNRA Full Length Protein-VLP (EDA-H52P8) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Asn 427 (Accession # P25101-1).
Description: Human EDNRB Full Length Protein-VLP (EDB-H52P7) is expressed from human 293 cells (HEK293). It contains AA Glu 27 - Ser 442 (Accession # P24530-1).
Description: Human SSTR2 Full Length Protein-VLP (SS2-H5216) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ile 369 (Accession # P30874-1).
Description: Human SSTR2 Full Length Protein-VLP (SS2-H5216) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ile 369 (Accession # P30874-1).
Description: Human SSTR2 Full Length Protein-VLP (SS2-H5216) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ile 369 (Accession # P30874-1).
Description: Human SSTR2 Full Length Protein-VLP (SS2-H5216) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ile 369 (Accession # P30874-1).
Description: Human GPRC5D Full Length Protein-VLP (GPD-H52P5) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Cys 300 (Accession # Q9NZD1-2).
Description: Human GPRC5D Full Length Protein (VLP) (GPD-H52P7) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Val 345 (Accession # Q9NZD1-1).
Description: Human ADORA2A Full Length Protein-VLP (ADA-H52P4) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ser 412 (Accession # P29274-1).
Description: Human Histone 3 (HIST1H3E), GenBank Accession No. NM_003532, a.a. 2-136(end) with N-terminal His-tag MW = 15.4 kDa, expressed in an E. coli expression system.
Description: Human Histone H1, also known as H1F0, (GenBank Accession No. NM_005318), a.a. 2-194(end) with N-terminal His-tag, MW = 21.7 kDa, expressed in an E. coli expression system.
Description: Human Histone H1, also known as H1F0, (GenBank Accession No. NM_005318), a.a. 2-194(end) with N-terminal His-tag, MW = 21.7 kDa, expressed in an E. coli expression system.
Recombinant HCV Protein (Full Length) (Baculovirus)
Description: Human S1PR5 Full Length Protein (VLP) (S15-H52P7) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Asp 398 (Accession # Q9H228-1).
Description: Human Histone 2A, GenBank Accession No. NM_033445, a.a. 2-130(end) with N-terminal His-tag, MW = 14.8 kDa, expressed in an E. coli expression system.
Description: Human Histone 2B, GenBank Accession No. NM_003528, a.a. 2-126(end) with N-terminal His-tag, MW = 14.6 kDa, expressed in an E. coli expression system. _x000D_
Description: Human Histone 2A, GenBank Accession No. NM_033445, a.a. 2-130(end) with N-terminal His-tag, MW = 14.8 kDa, expressed in an E. coli expression system.
Description: Human Histone 2B, GenBank Accession No. NM_003528, a.a. 2-126(end) with N-terminal His-tag, MW = 14.6 kDa, expressed in an E. coli expression system. _x000D_
Description: Protein tyrosine phosphatase PTP1B, also known as PTPN1 (GenBank accession number M31724), full length, (a.a. 1-436), with N terminal GST-tag, MW=76 kDa, expressed in an E. coli expression system.
Recombinant MeV Nucleocapsid Protein (Full Length)
Description: Human Claudin-1 Full Length Protein (VLP) (CL1-H52P6) is expressed from human 293 cells (HEK293). It contains AA Met 1 -Val 211 (Accession # O95832).
Description: Human Claudin-4 Full Length Protein (VLP) (CL4-H52P5) is expressed from human 293 cells (HEK293). It contains AA Met 1- Val 209 (Accession # O14493-1).
Description: Human CXCR4 Full Length Protein-VLP (CX4-H5219) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ser 352 (Accession # P61073-1).
Description: Human CXCR6 Full Length Protein (VLP) (CX6-H52P3) is expressed from human 293 cells (HEK293). It contains AA Ala 2 - Leu 342 (Accession # O00574-1).
Description: Human Claudin-18.1 Full Length Protein (VLP) (CL1-H52P3) is expressed from human 293 cells (HEK293). It contains AA Met 1- Val 261 (Accession # P56856-1).
Description: Human Claudin-18.2 Full Length Protein-VLP (CL2-H52P7) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Val 261 (Accession # P56856-2 ).
Description: Human JARID1B, also_x000D_ known as KDM5B and PLU-1 (GenBank_x000D_ Accession No. NM_006618), a.a. 2-_x000D_1544(end) with N-terminal FLAG-Avi-tag,_x000D_ MW=179 kDa, expressed in Sf9 cells via_x000D_ a baculovirus expression system.
Synthesis of Full-Size cDNA Infectious Clones of Soybean Mosaic Virus and Purposeful Identification of a Key Amino Acid within the Silencing Suppressor Hc-Professional
Soybean mosaic virus (SMV), which belongs to the Potyviridae, causes vital reductions in soybean yield and seed high quality. On this examine, each tag-free and reporter gene inexperienced fluorescent protein (GFP)-containing infectious clones for the SMV N1 pressure have been constructed by Gibson meeting and with the yeast homologous recombination system, respectively.
Each infectious clones are appropriate for agroinfiltration on the mannequin host benthamiana and present robust infectivity for the pure host soybean and several other different legume species. Each infectious clones have been seed transmitted and prompted typical virus signs on seeds and progeny crops. We used the SMV-GFP infectious clone to additional examine the function of key amino acids within the silencing suppressor helper component-proteinase (Hc-Professional).
Amongst twelve amino acid substitution mutants, the co-expression of mutant 2-with an Asparagine→Leucine substitution at place 182 of the FRNK (Phe-Arg-Asn-Lys) motif-attenuated viral signs and alleviated the host progress retardation brought on by SMV.
Furthermore, the Hc-Prom2 mutant confirmed stronger oligomerization than wild-type Hc-Professional. Taken collectively, the SMV infectious clones can be helpful for research of host-SMV interactions and useful gene characterization in soybeans and associated legume species, particularly by way of seed transmission properties. Moreover, the SMV-GFP infectious clone will even facilitate useful research of each virus and host genes in an benthamiana transient expression system.
Profiling of rice Cd-tolerant genes by way of yeast-based cDNA library survival screening
The bioaccumulation of cadmium (Cd) in crop and the next meals chain has aroused intensive considerations. Nevertheless, the underlying molecular mechanisms of plant Cd tolerance stay to be clarified from the viewpoint of novel candidate genes.
Right here we described a extremely environment friendly strategy for preliminary figuring out rice Cd-tolerant genes by way of the yeast-based cDNA library survival screening mixed with high-throughput sequencing technique. About 690 gene isoforms have been recognized as being Cd-tolerant candidates utilizing this shotgun strategy.
Among the many Cd-tolerant genes recognized, a number of classes of genes reminiscent of BAX inhibitor (BI), NAC transcription elements and Fast ALkalinization Elements (RALFs) have been of specific curiosity, and their perform of Cd tolerance was additional validated through heterologous expression, which urged that SNAC1, RALF12, OsBI-1 can confer Cd tolerance in yeast and tobacco leaves.
Relating to the genes concerned in ion transport, the validated Cd-tolerant heavy metal-associated area (HMAD) isoprenylated protein HIPP42 was significantly noteworthy. Additional elucidation of those genes related to Cd tolerance in rice will profit agricultural actions.
Single-Cell Transcriptomics of Immune Cells: Cell Isolation and cDNA Library Era for scRNA-Seq
Single-cell RNA-sequencing (scRNA-seq) allows a complete evaluation of the transcriptome of particular person cells by next-generation sequencing. ScRNA-seq gives an unbiased strategy to analyze the mobile heterogeneity and dynamics of various organic methods, together with the immune system. Optimization of the technical procedures carried out previous to RNA-seq evaluation is crucial to the success of a scRNA-seq experiment.
Right here, three main experimental procedures are described: (1) the isolation of immune CD8a+ T cells from main murine tissue, (2) the technology of single-cell cDNA libraries utilizing the 10× Genomics Chromium Controller and the Chromium Single Cell 3′ Answer, and (3) cDNA library high quality management. On this protocol, CD8a+ T cells are remoted from murine spleen tissue, however any cell kind of curiosity could be enriched and used for single-cell cDNA library technology and subsequent RNA-seq experiments.
-Human mRNA full length insert cDNA clone EUROIMAGE 1679989)
-Human mRNA full length insert cDNA clone EUROIMAGE 1623397)
-Human mRNA full length insert cDNA clone EUROIMAGE 901558)
-Human mRNA full length insert cDNA clone EUROIMAGE 1293175)
-Human mRNA full length insert cDNA clone EUROIMAGE 42353)
-Human mRNA full length insert cDNA clone EUROIMAGE 186647)
-Human mRNA full length insert cDNA clone EUROIMAGE 33793)
 Wolbachia Surface protein precursor (WSP, full length, his tag, >95%) for ELISA/Western)
 Hepatitis B Surface Antigen (HBsAg) pre-S protein (174-aa, ~25 kda, Adw2, full length, >95%, His-tag))
-Human full length insert cDNA YN63B03)
 ELISA Kit)
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 mouse monoclonal antibody, clone H398, PE)
 mouse monoclonal antibody, clone 5A18, Purified)
 mouse monoclonal antibody, clone H398, FITC)
 mouse monoclonal antibody, clone 11H40, Purified)
 mouse monoclonal antibody, clone H398, Biotin)
 mouse monoclonal antibody, clone H398, Purified)
 (full length) mouse monoclonal antibody, clone 111, Purified)
 rat monoclonal antibody, clone NM1, Azide Free)
 mouse monoclonal antibody, clone 4A2, Azide Free)
 mouse monoclonal antibody, clone 3A2, Azide Free)
 mouse monoclonal antibody, clone 4B4, Azide Free)
 (full length) mouse monoclonal antibody, clone 2D1, Purified)
 (full length) mouse monoclonal antibody, clone 8H8.5, Purified)
 (full length) mouse monoclonal antibody, clone 6C3.38, Purified)
 (full length) mouse monoclonal antibody, clone n.a, Purified)
 (full length) mouse monoclonal antibody, clone 2A10.8, Purified)
)
)
 mouse monoclonal antibody, clone H398, Low Endotoxin)
)
 (full length) mouse monoclonal antibody, clone 4B12, Azide Free)
)
, GST-tag)
 (full length) mouse monoclonal antibody, clone EBP-10, Purified)
 (Full length) mouse monoclonal antibody, clone 31A1067, Purified)
 (full length) mouse monoclonal antibody, clone 4C11C11C4, Aff - Purified)
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, FLAG-tag)
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 (full length) mouse monoclonal antibody, clone 4C1, Azide Free)
 (full length) mouse monoclonal antibody, clone 7A3, Aff - Purified)
 [His6])
 [His6])
 [His6])
 [His6])
 [His6])
 [His6])
 [His6])
 [His6])
 [His6])
 [His6])
 [His6])
 [His6])
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 (full length) mouse monoclonal antibody, clone 1C5-D9, Purified)
 Protein)
 [His])
)
 (full length) mouse monoclonal antibody, clone 2D4-1G9, Purified)
His)
)
 (Baculovirus))
)
)
 full length, GST-tag)
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)
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 [His])
 (E. coli))
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, Full Length, FLAG-tag)
 - Mouse RIKEN cDNA 2410091C18 gene (cDNA clone MGC:117993 IMAGE:4220568))
 - Mouse RIKEN cDNA 2400003C14 gene (cDNA clone MGC:6464 IMAGE:2631157))
 - Mouse RIKEN cDNA 6720467C03 gene (cDNA clone MGC:28237 IMAGE:3991990))
 - Mouse RIKEN cDNA 1700112C13 gene (cDNA clone MGC:107146 IMAGE:30284660))
 - Mouse RIKEN cDNA 9530077C05 gene (cDNA clone MGC:48244 IMAGE:3326047))
 - Mouse RIKEN cDNA 5830417C01 gene (cDNA clone MGC:61384 IMAGE:5702476))
 - Mouse RIKEN cDNA 6430701C03 gene (cDNA clone MGC:106253 IMAGE:4481856))
 - Mouse RIKEN cDNA 3830406C13 gene (cDNA clone MGC:68058 IMAGE:4020532))
 - Mouse RIKEN cDNA 2810047C21 gene (cDNA clone MGC:78216 IMAGE:30442538))
 - Mouse RIKEN cDNA 3830406C13 gene (cDNA clone MGC:36213 IMAGE:4194414))
 - Mouse RIKEN cDNA 0610007C21 gene (cDNA clone MGC:58396 IMAGE:6585654))
 - Mouse RIKEN cDNA 0610008C08 gene (cDNA clone MGC:27851 IMAGE:3489997))
 - Mouse RIKEN cDNA 2410091C18 gene (cDNA clone MGC:117993 IMAGE:4220568))
 - Mouse RIKEN cDNA 2400003C14 gene (cDNA clone MGC:6464 IMAGE:2631157))
 - Mouse RIKEN cDNA 6720467C03 gene (cDNA clone MGC:28237 IMAGE:3991990))
 - Mouse RIKEN cDNA 1700112C13 gene (cDNA clone MGC:107146 IMAGE:30284660))
 - Mouse RIKEN cDNA 9530077C05 gene (cDNA clone MGC:48244 IMAGE:3326047))
 - Mouse RIKEN cDNA 5830417C01 gene (cDNA clone MGC:61384 IMAGE:5702476))
 - Mouse RIKEN cDNA 6430701C03 gene (cDNA clone MGC:106253 IMAGE:4481856))
 - Mouse RIKEN cDNA 3830406C13 gene (cDNA clone MGC:68058 IMAGE:4020532))
 - Mouse RIKEN cDNA 2810047C21 gene (cDNA clone MGC:78216 IMAGE:30442538))
 - Mouse RIKEN cDNA 3830406C13 gene (cDNA clone MGC:36213 IMAGE:4194414))
 - Mouse RIKEN cDNA 0610007C21 gene (cDNA clone MGC:58396 IMAGE:6585654))
 - Mouse RIKEN cDNA 0610008C08 gene (cDNA clone MGC:27851 IMAGE:3489997))
 - Mouse RIKEN cDNA 9530077C05 gene (cDNA clone MGC:48244 IMAGE:3326047))
 - Mouse RIKEN cDNA 2400003C14 gene (cDNA clone MGC:6464 IMAGE:2631157))
 - Mouse RIKEN cDNA 6720467C03 gene (cDNA clone MGC:28237 IMAGE:3991990))
 - Mouse RIKEN cDNA 6030419C18 gene (cDNA clone MGC:106198 IMAGE:6468423))
 - Mouse RIKEN cDNA 1700112C13 gene (cDNA clone MGC:107146 IMAGE:30284660))
 - Mouse RIKEN cDNA 2610016C23 gene (cDNA clone MGC:29347 IMAGE:5036908))
 - Mouse RIKEN cDNA 2410091C18 gene (cDNA clone MGC:117993 IMAGE:4220568))
 - Mouse RIKEN cDNA 0610007C21 gene (cDNA clone MGC:58396 IMAGE:6585654))
 - Mouse RIKEN cDNA 0610008C08 gene (cDNA clone MGC:27851 IMAGE:3489997))
 - Mouse RIKEN cDNA 5830417C01 gene (cDNA clone MGC:61384 IMAGE:5702476))
 - Mouse RIKEN cDNA 2810047C21 gene (cDNA clone MGC:78216 IMAGE:30442538))
 - Mouse RIKEN cDNA 3830406C13 gene (cDNA clone MGC:36213 IMAGE:4194414))
 - Mouse RIKEN cDNA 6430701C03 gene (cDNA clone MGC:106253 IMAGE:4481856))
 - Mouse RIKEN cDNA 3830406C13 gene (cDNA clone MGC:68058 IMAGE:4020532))
 - Mouse RIKEN cDNA C730004C24 gene (cDNA clone MGC:102487 IMAGE:5132591))
 - Mouse RIKEN cDNA C730048C13 gene (cDNA clone MGC:54808 IMAGE:5044850))
 - Mouse RIKEN cDNA 6720467C03 gene (cDNA clone MGC:28237 IMAGE:3991990), (10ug))
 - Mouse RIKEN cDNA 2410091C18 gene (cDNA clone MGC:117993 IMAGE:4220568), (10ug))
 - Mouse RIKEN cDNA 1700112C13 gene (cDNA clone MGC:107146 IMAGE:30284660), (10ug))
 - Mouse RIKEN cDNA 3000004C01 gene (cDNA clone MGC:67548 IMAGE:6401536), (10ug))
 - Mouse RIKEN cDNA 4732418C07 gene (cDNA clone MGC:66557 IMAGE:6408754), (10ug))
 - Mouse RIKEN cDNA 4930507C10 gene (cDNA clone MGC:27771 IMAGE:3154701), (10ug))
 - Mouse RIKEN cDNA 2610016C23 gene (cDNA clone MGC:29347 IMAGE:5036908), (10ug))
 - Mouse RIKEN cDNA 2410187C16 gene (cDNA clone MGC:63069 IMAGE:1514917), (10ug))
 - Mouse RIKEN cDNA 6430701C03 gene (cDNA clone MGC:106253 IMAGE:4481856), (10ug))
 - Mouse RIKEN cDNA 0610008C08 gene (cDNA clone MGC:27851 IMAGE:3489997), (10ug))
 - Mouse RIKEN cDNA 2810047C21 gene (cDNA clone MGC:78216 IMAGE:30442538), (10ug))
 - Mouse RIKEN cDNA 2410001C21 gene (cDNA clone MGC:8144 IMAGE:3589578), (10ug))
 - Mouse RIKEN cDNA 5530400C23 gene (5530400C23Rik))
 - Mouse RIKEN cDNA 4930447C04 gene (4930447C04Rik))
 - Mouse RIKEN cDNA 5330417C22 gene (5330417C22Rik))
 - Mouse RIKEN cDNA 1700084C01 gene (1700084C01Rik))
 - Mouse RIKEN cDNA 4921509C19 gene (4921509C19Rik))
 - Mouse RIKEN cDNA 4933433C11 gene (4933433C11Rik))
 - Mouse RIKEN cDNA 1700037C18 gene (1700037C18Rik))
 - Mouse RIKEN cDNA 4930550C14 gene (4930550C14Rik))
 - Mouse RIKEN cDNA 6820408C15 gene (6820408C15Rik))
 - Mouse RIKEN cDNA 1110007C09 gene (1110007C09Rik))
 - Mouse RIKEN cDNA 4921511C04 gene (4921511C04Rik))
 - Mouse RIKEN cDNA 6030419C18 gene (6030419C18Rik))
 - Mouse RIKEN cDNA 2700062C07 gene (2700062C07Rik))
 - Mouse RIKEN cDNA 1700129C05 gene (1700129C05Rik))
 - Mouse RIKEN cDNA 2210407C18 gene (2210407C18Rik))
 - Mouse RIKEN cDNA 1700001C02 gene (1700001C02Rik))
 - Mouse RIKEN cDNA 2210010C04 gene (2210010C04Rik))
 - Mouse RIKEN cDNA 1700018C11 gene (1700018C11Rik))
 - Mouse RIKEN cDNA 2900092C05 gene (2900092C05Rik))
 - Mouse RIKEN cDNA 4930549C01 gene (4930549C01Rik))
 - Mouse RIKEN cDNA 4930547C10 gene (4930547C10Rik))
 - Mouse RIKEN cDNA 9230110C19 gene (9230110C19Rik))
 - Mouse RIKEN cDNA 4933416C03 gene (4933416C03Rik))
 - Mouse RIKEN cDNA 2310050C09 gene (2310050C09Rik))
 - Mouse RIKEN cDNA 4930578C19 gene (4930578C19Rik))
 - Mouse RIKEN cDNA 4930562C15 gene (4930562C15Rik))
 - Mouse RIKEN cDNA 4931408C20 gene (4931408C20Rik))
 - Mouse RIKEN cDNA 1700016C15 gene (1700016C15Rik))
 - Mouse RIKEN cDNA 5730559C18 gene (5730559C18Rik))
 - Mouse RIKEN cDNA 9530077C05 gene (9530077C05Rik))
 - Mouse RIKEN cDNA 4922501C03 gene (4922501C03Rik))
 - Mouse RIKEN cDNA C730004C24 gene (cDNA clone MGC:102487 IMAGE:5132591))
 - Mouse RIKEN cDNA C730048C13 gene (cDNA clone MGC:54808 IMAGE:5044850))
 - Mouse RIKEN cDNA 2410091C18 gene (cDNA clone MGC:117993 IMAGE:4220568))
 - Mouse RIKEN cDNA 2400003C14 gene (cDNA clone MGC:6464 IMAGE:2631157))
 - Mouse RIKEN cDNA 6720467C03 gene (cDNA clone MGC:28237 IMAGE:3991990))
 - Mouse RIKEN cDNA 1700112C13 gene (cDNA clone MGC:107146 IMAGE:30284660))
 - Mouse RIKEN cDNA 9530077C05 gene (cDNA clone MGC:48244 IMAGE:3326047))
 - Mouse RIKEN cDNA 5830417C01 gene (cDNA clone MGC:61384 IMAGE:5702476))
 - Mouse RIKEN cDNA 6430701C03 gene (cDNA clone MGC:106253 IMAGE:4481856))
 - Mouse RIKEN cDNA 3830406C13 gene (cDNA clone MGC:68058 IMAGE:4020532))
 - Mouse RIKEN cDNA 2810047C21 gene (cDNA clone MGC:78216 IMAGE:30442538))
 - Mouse RIKEN cDNA 3830406C13 gene (cDNA clone MGC:36213 IMAGE:4194414))
 - Mouse RIKEN cDNA 0610007C21 gene (cDNA clone MGC:58396 IMAGE:6585654))
 - Mouse RIKEN cDNA 0610008C08 gene (cDNA clone MGC:27851 IMAGE:3489997))
 - Mouse RIKEN cDNA 5530400C23 gene (5530400C23Rik))
 - Mouse RIKEN cDNA 4930447C04 gene (4930447C04Rik))
 - Mouse RIKEN cDNA 5330417C22 gene (5330417C22Rik))
 - Mouse RIKEN cDNA 1700084C01 gene (1700084C01Rik))
 - Mouse RIKEN cDNA 4921509C19 gene (4921509C19Rik))
 - Mouse RIKEN cDNA 4933433C11 gene (4933433C11Rik))
 - Mouse RIKEN cDNA 1700037C18 gene (1700037C18Rik))
 - Mouse RIKEN cDNA 4930550C14 gene (4930550C14Rik))
 - Mouse RIKEN cDNA 6820408C15 gene (6820408C15Rik))
 - Mouse RIKEN cDNA 1110007C09 gene (1110007C09Rik))
 - Mouse RIKEN cDNA 4921511C04 gene (4921511C04Rik))