Identification of salinity responsive genes in lavender by way of cDNA-AFLP
Presently, a world demand exists forlavender as a big medicinal plant and supply of important oils. Freshwater and arable lands are two main elements that inhibit intensive farming of medicinal crops in Iran. Saline water from seas and salty soil could also be new sources for agricultural use, particularly for medicinal crops.
We sought to increase our data of the Lavandulaangustifolia genome and molecular foundation of its salinity tolerance through the use of cDNA amplified fragment size polymorphism (cDNA-AFLP) to analyze the adjustments in plant transcriptomes in response to NaCl.
All recognized transcript derived fragments (TDF) have been assigned as novel angustifolia genes associated to sign transduction, regulation of gene expression, different splicing, autophagy, and secondary metabolite biosynthesis. qRT-PCR evaluation of the TDFs in response to completely different concentrations of NaCl revealed varied ranges of mRNA of the recognized genes on this plant.
Our findings supplied main insights into the molecular response of angustifolia to salinity.
Description: A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa.
Description: Quantitativesandwich ELISA kit for measuring Human vascular endothelial cell growth factor E, VEGF-E in samples from serum, urine, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Human vascular endothelial cell growth factor E, VEGF-E ELISA Kit
Description: Quantitativesandwich ELISA kit for measuring Human vascular endothelial cell growth factor E, VEGF-E in samples from serum, urine, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: Qualitative indirect ELISA kit for measuring Human Hepatitis E virus antibody (IgG) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Qualitative indirect ELISA kit for measuring Human Hepatitis E virus antibody(IgG) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Qualitative indirect ELISA kit for measuring Human Hepatitis E virus antibody (IgM) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Qualitative indirect ELISA kit for measuring Human Hepatitis E virus antibody(IgM) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Hepatitis E virus genotype 1 Capsid protein (ORF2)
Description: Primary antibody against VEGF-A(VEGF/1063), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL
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Identification and characterization of a cDNA encoding a gametocyte-specific protein of the avian coccidial parasite Eimerianecatrix
Gametocyte proteins of Eimeria spp. are important elements of the oocyst wall, and a few of these proteins have been analysed to establish targets of transmission-blocking vaccines in opposition to avian coccidiosis. Within the current examine, a cDNA from E. necatrix gametocytes was cloned and sequenced. The cDNA is 1,473 bp in size and encodes a 490-amino-acid protein containing a tyrosine-serine (Tyr/Ser)-rich area and a proline-methionine (Professional/Met)-rich area.
A quantitative real-time PCR (qPCR) evaluation confirmed that the cDNA is expressed solely throughout gametogenesis. A fraction containing the Tyr/Ser-rich area (rEnGAM59) was expressed in Escherichia coli BL21 (DE3) cells. Immunoblotting confirmed that rEnGAM59 was acknowledged by the serum of convalescent chickens after an infection with E. necatrix, and that an anti-rEnGAM59 antibody acknowledged a ∼59 kDa protein and
two different proteins (∼35 kDa and ∼33 kDa) in gametocyte extracts. An immunofluorescence assay confirmed that the anti-rEnGAM59 antibody acknowledged wall-forming our bodies within the macrogametocytes and oocyst partitions. An in vivo vaccination and problem trial was carried out to check the potential utility of rEnGAM59 as a vaccine.
Immunized chickens carried out higher than the unimmunized and challenged (constructive management) chickens. The intestinal lesion scores have been considerably decrease within the immunized teams than within the constructive management group (P < 0.05). In distinction, the physique weight positive aspects (BWG) have been considerably larger within the immunized teams than within the constructive management group (P < 0.05). There have been no vital variations within the lesion scores and BWG between the teams immunized with rEnGAM59 protein or with stay oocysts (P > 0.05). Chickens immunized with rEnGAM59 protein had a considerably larger antigen-specific serum IgY response (P < 0.05). rEnGAM59 protein can be utilized as candidate antigen to develop a recombinant coccidiosis vaccine.
Reverse genetics of rotaviruses: Generation of recombinant human rotaviruses from just 11 cDNAs encoding the rotavirus genome
A completely plasmid-based reverse genetics system for animal rotavirus was established very lately. We improved the reverse genetics system to generate recombinant rotavirus by transfecting solely 11 T7 plasmids for its 11 genes below the situation of accelerating the ratio (3- or 5-fold) of the cDNA plasmids for NSP2 and NSP5 genes (11-plasmid system).
Using this extremely environment friendly system, we engineered the primary infectious recombinant rotaviruses harboring fluorescent (EGFP and mCherry) protein genes. Along with these recombinant animal viruses, the first infectious recombinant human rotavirus (pressure KU (G1P[8])) was additionally generated with the 11-plasmid system with some modifications.
The supply of recombinant human rotaviruses will present a genetic platform for a greater understanding of the replication, pathogenicity, and different organic traits of this medically vital virus and allow the rational improvement of next-generation human rotavirus vaccines.
Role of T cell immune response cDNA 7 on the pathology of acute graft-versus-host disease
Activation of T lymphocytes is the initiating factor of the occurrence of acute graft-versus-host disease (aGVHD), and cytotoxic T lymphocyte antigen-4 (CTLA-4) is the inhibitory receptor for activating T cells. T cell immune response cDNA 7 (TIRC7) is considered an upstream regulator of CTLA-4; however, little is understood regarding the effects of TIRC7 on the regulation of CTLA-4 in aGVHD. The purpose of the present study was to evaluate the regulatory effects of TIRC7 on aGVHD, mainly in the pathology.
Recipient mice were exposed to a preconditioning dose of 7.5 Gy irradiation on the day of the transplantation and were divided into the following groups: Blank control group, bone marrow transplantation control group, total body irradiation group, mild-moderate aGVHD group and severe aGVHD group. According to the different administration of CTLA-4 and TIRC7 monoclonal antibodies, the mild-moderate and severe aGVHD groups were randomly divided into the hematopoietic stem cell transplantation (HSCT) and HSCT + CTLA-4/TIRC7 groups.
Recipient mice were sacrificed at different time points post-HSCT for histopathological analysis by hematoxylin and eosin staining. Compared with the control and other experimental groups, the mice in the combined CTLA-4 and TIRC7 group exhibited ameliorated pathological injury, and lower pathology scores of the liver, lung and intestine. These data revealed that intraperitoneal injection of anti-TIRC7 and/or anti-CTLA-4 monoclonal antibody into mice could effectively alleviate the severity of aGVHD.
Construction of a cDNA expression library in a binary vector using a nicking enzyme
Ligation-independent cloning (LIC), such as Gibson Assembly, tends to produce clones without an insert, depending on the sequences present at the ends of linearized vectors. We used a nicking enzyme-mediated LIC (NE-LIC) method to construct a cDNA library in a binary vector pER8. Prior to constructing the cDNA library, pilot experiments were carried out, in which the GUS coding sequence was cloned into pER8 using NE-LIC.
Approximately 12% of input vector DNAs were converted to plasmids carrying a GUS insert, and no plasmids without an insert were detected, indicating that this strategy is highly effective for cloning with the binary vector pER8. Therefore, NE-LIC was adopted to construct a cDNA library in pER8, by using cDNA that was PCR-amplified from a library constructed in another vector. As a result, a cDNA library in pER8 was successfully constructed.
During library construction, it is important to exclude plasmids without an insert, since contamination from plasmids without inserts decreases the efficiency of screening. Therefore, NE-LIC is useful for the construction of cDNA libraries.
Description: A sandwich quantitative ELISA assay kit for detection of Human Complement Factor B (CFB) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Complement Factor B (CFB) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Complement Factor B (CFB) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Complement Factor B (CFB) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Complement Factor B (CFB) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Complement Factor B (CFB) in samples from serum, plasma or other biological fluids.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CFB (Center). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody against CFB. Recognizes CFB from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:200-1:500
Description: A polyclonal antibody against CFB. Recognizes CFB from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB
Description: A polyclonal antibody against Cfb. Recognizes Cfb from Mouse, Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human CFB / Complement Factor B . This antibody is tested and proven to work in the following applications:
Description: This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.
Description: This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.
Complement Factor B (CFB) Polyclonal Antibody (Human)
Description: A polyclonal antibody against CFB. Recognizes CFB from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against CFB. Recognizes CFB from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against Cleaved-CFB (K260). Recognizes Cleaved-CFB (K260) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000
Description: A polyclonal antibody against Cfb. Recognizes Cfb from Mouse. This antibody is FITC conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against Cfb. Recognizes Cfb from Mouse. This antibody is Biotin conjugated. Tested in the following application: ELISA
Complement Factor B (CFB) Polyclonal Antibody (Human), APC
