Extraction of high-quality tissue-specific RNA from London plane timber (Platanusacerifolia), permitting the event of a female inflorescence cDNA library
The London plane tree (PlatanusacerifoliaWilld.) has worldwide significance as an metropolis landscaping tree and is the subject of genetic-improvement purposes for productive sterility, sickness and/or insect resistance. Molecular analysis strategies are important to such purposes, nevertheless may be impeded by specific difficulties encountered all through nucleic acid isolation.
An in depth RNA isolation and purification protocol, based on established cetyltrimethyl-ammonium bromide (CTAB) extraction strategies blended with additional purification steps using butanol and the ionic detergent CTAB, which overcomes these points throughout the woody species P. acerifolia, was carried out. Briefly, phenolic compounds are certain to soluble polyvinylpyrrolidone after which separated out by means of LiCl precipitation of the RNA.
Subsequently, protein- and carbohydrate-contaminants are eradicated by chloroform partitioning adopted by LiCl-mediated precipitation. The following isolates of RNA have been found to be of sufficient top quality for worthwhile use in reverse transcription PCR analysis.
Furthermore, RNA isolates from female inflorescences have been used for the event of a cDNA library. This library was found to incorporate quite a few full-length cDNA clones of MADS-box genes, in keeping with the library being marketing consultant of inflorescence expression profiles.
Description: A Monoclonal antibody against Human LILRB2 (monoclonal) (M01). The antibodies are raised in mouse and are from clone 1D4. This antibody is applicable in WB, E
Description: A Rabbit polyclonal antibody against Human, Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2). This antibody is labeled with APC.
Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2) Polyclonal Antibody (Human, Mouse), Cy3
Description: A Rabbit polyclonal antibody against Human, Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2). This antibody is labeled with Cy3.
Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2) Polyclonal Antibody (Human, Mouse), HRP
Description: A Rabbit polyclonal antibody against Human, Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2). This antibody is labeled with HRP.
Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2) Polyclonal Antibody (Human, Mouse), PE
Description: A Rabbit polyclonal antibody against Human, Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2). This antibody is labeled with PE.
Description: A polyclonal antibody against LILRB2. Recognizes LILRB2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200
Description: A polyclonal antibody against LILRB2. Recognizes LILRB2 from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000
Description: A polyclonal antibody against LILRB2. Recognizes LILRB2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100
Description: A Rabbit polyclonal antibody against Human, Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2). This antibody is labeled with FITC.
Description: A Rabbit polyclonal antibody against Human, Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2). This antibody is labeled with APC-Cy7.
Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2) Polyclonal Antibody (Human, Mouse), Biotinylated
Description: A Rabbit polyclonal antibody against Human, Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2). This antibody is labeled with Biotin.
Description: Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) is also known as CD85 antigen-like family member D (CD85d), Immunoglobulin-like transcript 4 (ILT-4), Monocyte / macrophage immunoglobulin-like receptor 10 (MIR-10), which is a member of the the subfamily B class of LIR receptors. LILRB2 is receptor for class I MHC antigens. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB2 competes with CD8A for binding to class I MHC antigens. LILRB2 / CD85d inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
Description: Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) is also known as CD85 antigen-like family member D (CD85d), Immunoglobulin-like transcript 4 (ILT-4), Monocyte / macrophage immunoglobulin-like receptor 10 (MIR-10), which is a member of the the subfamily B class of LIR receptors. LILRB2 is receptor for class I MHC antigens. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB2 competes with CD8A for binding to class I MHC antigens. LILRB2 / CD85d inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
Description: Members of the immunoglobulin-like transcript (ILT) family are activating and inhibitory immunoreceptors whose genes are located same locus that encodes killer cell Ig-like receptors (KIR). Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2 (LIR-2) is a type I transmembrane protein. LIR-2 is expressed primarily on monocytes and dendritic cells (DC). Human LIR-2 is produced as a 598 amino acino acid precursor including a 21 aa signal sequence, a 440 aa extracellular domain (ECD), a 21 aa transmenbrane segment, and a 116 aa cytoplasmic domain. LIR-2 binds to Classical MHCI proteins. Ligation of LIR-2 incluces Tyr phosphorylation within its cytoplasmic ITIMs, a requirement for association with SHP-1. LIR-2 mediates tolerogenic DC-induced CD4+ T cell energy in vitro and in vivo.
Description: This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.
Description: This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.
Description: This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.
Lilrb3 (Myc-DDK-tagged) - Mouse leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3 (cDNA clone MGC:30225
Transcriptome analysis of leaf tissue from Bermudagrass (Cynodondactylon) using a normalised cDNA library
A normalised cDNA library was constructed from Bermudagrass to attain notion into the transcriptome of Cynodondactylon L. A whole of 15 588 high-top quality expressed sequence tags (ESTs) from the cDNA library have been subjected to The Institute for Genomic Evaluation Gene Indices clustering devices to supply a unigene set.
A whole of 9414 unigenes have been obtained from the high-quality ESTs and solely 39.6% of the high-quality ESTs have been redundant, indicating that the normalisation course of was environment friendly. An enormous-scale comparative genomic analysis of the unigenes was carried out using publicly obtainable devices, paying homage to BLAST, InterProScan and Gene Ontology. The unigenes have been moreover subjected to a look for EST-derived simple sequence repeats (EST-SSRs) and conserved-intron scanning primers (CISPs), which can be useful as DNA markers.
Although the candidate EST-SSRs and CISPs found throughout the present look at ought to be empirically examined, they’re anticipated to be useful as DNA markers for lots of features, along with comparative genomic analysis of grass species, by benefit of their very important similarities to EST sequences from completely different grasses. Thus, knowledge of Cynodon ESTs will empower turfgrass evaluation by providing homologues for genes that are thought to confer very important options in numerous crops.
Prolonged-read cDNA Sequencing Permits a ‘Gene-Like’ Transcript Annotation of Transposable Elements
Transcript-based annotations of genes facilitate every genome-wide analyses and detailed single locus evaluation. In distinction, transposable issue (TE) annotations are rudimentary, consisting of data solely on TE location and type. The repetitiveness and restricted annotation of TEs prevents the facility to inform aside between doubtlessly helpful expressed elements and degraded copies.
To reinforce genome-wide TE bioinformatics, we carried out long-read sequencing of cDNAs from Arabidopsis thaliana strains poor in quite a few layers of TE repression. These uniquely-mapping transcripts have been used to ascertain the set of TEs able to generate polyadenylated RNAs and create a model new transcript-based annotation of TEs that we’ve now layered upon the current high-quality neighborhood regular annotation.
We used this annotation to chop again the bioinformatic complexity associated to multi-mapping reads from short-read RNA-seq experiments, and we current that this enchancment is expanded in a TE-rich genome paying homage to maize. Our TE annotation moreover permits the testing of specific standing hypotheses throughout the TE self-discipline.
We show that incorrect TE splicing would not set off small RNA manufacturing, and the cell further strongly targets DNA methylation to TEs which have the potential to make mRNAs. This work provides a model new transcript-based TE annotation for Arabidopsis and maize, which serves as a blueprint to chop again the bioinformatic complexity associated to repetitive TEs in any organism.
Identification of Avramr1 from Phytophthora infestans utilizing lengthy learn and cDNA pathogen-enrichment sequencing (PenSeq)
Potato late blight, brought on by the oomycete pathogen Phytophthora infestans, considerably hampers potato manufacturing. Not too long ago, a brand new Resistance to Phytophthora infestans (Rpi) gene, Rpi-amr1, was cloned from a wild Solanum species, Solanum americanum. Identification of the corresponding acknowledged effector (Avirulence or Avr) genes from P. infestans is essential to elucidating their naturally occurring sequence variation, which in flip informs the potential sturdiness of the cognate late blight resistance. To establish the P. infestans effector acknowledged by Rpi-amr1, we screened accessible RXLR effector libraries and used lengthy learn and cDNA pathogen-enrichment sequencing (PenSeq) on 4 P. infestans isolates to discover the untested effectors.
Utilizing single-molecule real-time sequencing (SMRT) and cDNA PenSeq, we recognized 47 extremely expressed effectors from P. infestans, together with PITG_07569, which triggers a extremely particular cell loss of life response when transiently coexpressed with Rpi-amr1 in Nicotiana benthamiana, suggesting that PITG_07569 is Avramr1.
Right here we exhibit that lengthy learn and cDNA PenSeq permits the identification of full-length RXLR effector households and their expression profile. This research has revealed key insights into the evolution and polymorphism of a posh RXLR effector household that’s related to the popularity by Rpi-amr1.
An revolutionary genosensor for the monitoring of Leishmania spp sequence utilizing binding of pDNA to cDNA primarily based on Cit-AgNPs
Leishmaniasis thought-about as probably the most essential epidemic-prone illnesses in keeping with the World Well being Group. Early diagnoses and remedy of Leishmania an infection is a superb problem since, it has no symptom and is resistance to medication. Subsequently, there’s an pressing want for delicate and exact detection of this pathogen.
On this research, a brand new methodology was developed for optical biosensing of Leishmania spp sequence primarily based on hybridization of Citrate capped Ag nanoparticles bonded to particular single stranded DNA probe of Leishmania spp.
Aggregation of the Citrate capped Ag nanoparticles within the existence or lack of a cDNA sequence of Leishmania, trigger eye catching and appreciable vital alter within the UV-vis. The obtained low restrict of quantification (LLOQ) of was achieved as 1ZM. Based mostly on experimental ends in optimum situations, fast bioanalysis of Leishmania spp sequence was carried out (2 min). So, this probe can be utilized for the medical analysis of this pathogen and an infection illness.
 (M01), Clone: 1D4)
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 Polyclonal Antibody (Human, Mouse))
 Polyclonal Antibody (Human, Mouse), APC)
 Polyclonal Antibody (Human, Mouse), Cy3)
 Polyclonal Antibody (Human, Mouse), HRP)
 Polyclonal Antibody (Human, Mouse), PE)
 Polyclonal Antibody (Human, Mouse), FITC)
 Polyclonal Antibody (Human, Mouse), APC-Cy7)
 Polyclonal Antibody (Human, Mouse), Biotinylated)
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 - Mouse leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3 (cDNA clone MGC:30225)
 - Mouse leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3 (cDNA clone MGC:30225, (10ug))
 - Mouse leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3 (cDNA clone MGC:30225)
 - Rat GTP-binding protein 8 (putative) (Gtpbp8), (10 ug))
 - Rat GTP-binding protein 8 (putative) (Gtpbp8), (10 ug))
 - Mouse GTP binding protein 5 (cDNA clone MGC:36295 IMAGE:4483063))
 - Mouse GTP-binding protein 8 (putative) (Gtpbp8), transcript variant 1)
 - Mouse GTP-binding protein 8 (putative) (Gtpbp8), transcript variant 2)
 - Mouse GTP binding protein 5 (cDNA clone MGC:36295 IMAGE:4483063))
 - Mouse GTP binding protein 5 (cDNA clone MGC:36295 IMAGE:4483063))
 - Mouse GTP-binding protein 8 (putative) (Gtpbp8), transcript variant 1)
 - Mouse GTP-binding protein 8 (putative) (Gtpbp8), transcript variant 2)
 - Mouse GTP binding protein 5 (cDNA clone MGC:36295 IMAGE:4483063), (10ug))
 - Mouse GTP binding protein 5 (cDNA clone MGC:36295 IMAGE:4483063))
)
 (AP))
 (PE))
 (APC))
 (FITC))
 (Biotin))
 - Mouse GTP binding protein 5 (cDNA clone MGC:36295 IMAGE:4483063), 200ul, >10^7 TU/mL)
 (MaxLight 405))
 (MaxLight 490))
 (MaxLight 550))
 (MaxLight 650))
 (MaxLight 750))
 - Mouse GTP binding protein 5 (cDNA clone MGC:36295 IMAGE:4483063), 200ul, >10^7 TU/mL)
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 (Azide free) (HRP))
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